About this book RNA-protein interactions play a vital role in gene transcription and protein expression. Contents 1. Applications of chemically synthesized RNA; 2. Affinity methods for isolating RNA-binding proteins; 6. A three-hybrid system to detect and analyse RNA-protein interactions in vivo; 9. In vivo selection of specific RNA-binding polypeptides using a transcription anti-termination reporter assay; Foot-printing and modification-interference analysis of binding sites on RNA; In vitro selection of nucleic acid ligands Customer Reviews Review this book.
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Schoenberg, D. Hogg, J. RNA 13 , — Leppek, K. An optimized streptavidin-binding RNA aptamer for purification of ribonucleoprotein complexes identifies novel ARE-binding proteins. Nucleic Acids Res. Lee, H. USA , — Kretz, M. Nature , — Siprashvili, Z. Sutherland, B. Utility of formaldehyde cross-linking and mass spectrometry in the study of protein-protein interactions. Mass Spectrom. Li, X. Genome-wide mapping of cellular protein-RNA interactions enabled by chemical crosslinking.
Genomics Proteomics Bioinformatics 12 , 72—78 Beckmann, B. RNA interactome capture in yeast. Methods , 82—92 Sugimoto, Y. Genome Biol. Kim, B. Methods , 3—11 Meisenheimer, K. Photocross-linking of nucleic acids to associated proteins. Hoffman, E. Formaldehyde crosslinking: a tool for the study of chromatin complexes.
Chu, C. Cell 44 , — Simon, M. The genomic binding sites of a noncoding RNA. McHugh, C. Methods Mol. Hacisuleyman, E. Topological organization of multichromosomal regions by the long intergenic noncoding RNA Firre. Zeng, F. Tsai, B. Quantitative profiling of in vivo —assembled RNA-protein complexes using a novel integrated proteomic approach.
Proteomics 10 , M Methods — , 93— Parrott, A. Systematic discovery of Xist RNA binding proteins. Google Scholar. Kim, D. Probing nuclear pore complex architecture with proximity-dependent biotinylation. An improved smaller biotin ligase for BioID proximity labeling. Cell 27 , — Roux, K. A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells.
Ramanathan, M. RNA—protein interaction detection in living cells. Methods 15 , — Bantscheff, M. Quantitative mass spectrometry in proteomics: a critical review. Comprehensive analysis of mass spectrometry methods used in proteomics. Butter, F. Unbiased RNA-protein interaction screen by quantitative proteomics. Mellacheruvu, D. The CRAPome: a contaminant repository for affinity purification—mass spectrometry data.
Methods 10 , — Tsai, T. Preprocessing and analysis of LC-MS-based proteomic data. OpenMS: a flexible open-source software platform for mass spectrometry data analysis. Methods 13 , — This paper presents a common platform with ready-made workflows allowing users to perform reproducible mass spectrometry analysis with ease. Cox, J. Andromeda: a peptide search engine integrated into the MaxQuant environment. Proteome Res. West, J. Cell 55 , — Yang, X. The dataset for protein-RNA binding affinity.
Protein Sci. Smith, K. A mixed photoproduct of uracil and cysteine 5-S-cysteinehydrouracil. A possible model for the in vivo cross-linking of deoxyribonucleic acid and protein by ultraviolet light. Biochemistry 5 , — Goddard, J. Studies on the inactivation of tobacco mosaic virus by ultraviolet light. Choi, Y.
USA 81 , — Background Citations. Methods Citations. Citation Type. Has PDF. Publication Type. More Filters. Identification and cell lineage of individual neural precursors in the Drosophila CNS. Trends in Neurosciences. Cell and Tissue Research. Highly Influenced.
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